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Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of <t>HiTRACE,</t> RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.
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Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of <t>HiTRACE,</t> RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.
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Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of <t>HiTRACE,</t> RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.
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Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of <t>HiTRACE,</t> RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.
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Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of <t>HiTRACE,</t> RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.
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Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of HiTRACE, RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.

Journal: RNA

Article Title: Folding heterogeneity in the essential human telomerase RNA three-way junction

doi: 10.1261/rna.077255.120

Figure Lengend Snippet: Data-guided RNA secondary structure prediction of medaka and human CR4/5 domain. SHAPE (1M7) reactivity data were used as weights to guide RNA structure prediction for medaka (A) and human (B) CR4/5 domains. Using the Biers package of HiTRACE, RNAstructure models of each RNA domain were calculated with 100 bootstrap replicates, while varying the SHAPE slope parameter in intervals of 0.2 kcal/mol. The abundance of each helical RNA element (Confidence) derives from the bootstrap replicates and is plotted for each respective value of SHAPE slope. Ref. Pin refers to Reference hairpin.

Article Snippet: SHAPE-guided predictions were performed with the HiTRACE MATLAB package.

Techniques: RNA Structure Prediction